Linked Life Data

21232939

Source:http://linkedlifedata.com/resource/pubmed/id/21232939

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-1-31
pubmed:abstractText
A new glutamic acid independent poly-?-glutamic acid (?-PGA) producing strain, which was identified as Bacillus amyloliquefaciens LL3 by analysis of 16S rDNA and gyrase subunit A gene (gyrA), was isolated from fermented food. The product had a molecular weight of 470, 801 and l-glutamate monomer content of 98.47%. The pre-optimal medium, based on single-factor tests and orthogonal design, contained 50 g/L sucrose, 2g/L (NH(4))(2)SO(4), 0.6g/L MgSO(4), and provided well-balanced changes in processing parameters and a ?-PGA yield of 4.36 g/L in 200 L system. The ?-PGA synthetase genes pgsBCA were cloned from LL3, and successfully expressed by pTrcLpgs vector in Escherichia coli JM109, resulting the synthesis of ?-PGA without glutamate. This study demonstrates the designedly improved yield of ?-PGA in 200 L system and the first report of pgsBCA from glutamic acid independent strain, which will benefit the metabolized mechanism investigation and the wide-ranging application of ?-PGA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1873-2976
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4251-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Glutamic acid independent production of poly-?-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes.
pubmed:affiliation
Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin 300071, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't