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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
1991-1-8
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pubmed:databankReference |
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pubmed:abstractText |
We report the cloning and sequencing of a 1225-base pair cDNA encoding an abundant protein from mouse vas deferens. An open reading frame of 948 nucleotides encodes a protein of 316 amino acids with a calculated mass of 35,965 Da. A high degree of homology was found between this protein and members of the aldo-keto reductase superfamily, especially aldose reductase from human placenta (82%) and from rat lens (80%), suggesting that it could be an aldose reductase. Castration resulted in a marked decrease in the level of the 1.4 kilobase mRNA coding for the 35,965 protein, whereas administration of testosterone to castrated males resulted in a marked increase. Southern hybridization analysis of mouse genomic DNA revealed relatively simple patterns of bands.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19932-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2123194-Aldehyde Reductase,
pubmed-meshheading:2123194-Amino Acid Sequence,
pubmed-meshheading:2123194-Animals,
pubmed-meshheading:2123194-Base Sequence,
pubmed-meshheading:2123194-Cloning, Molecular,
pubmed-meshheading:2123194-DNA,
pubmed-meshheading:2123194-Humans,
pubmed-meshheading:2123194-Lens, Crystalline,
pubmed-meshheading:2123194-Male,
pubmed-meshheading:2123194-Mice,
pubmed-meshheading:2123194-Molecular Sequence Data,
pubmed-meshheading:2123194-Orchiectomy,
pubmed-meshheading:2123194-Placenta,
pubmed-meshheading:2123194-Proteins,
pubmed-meshheading:2123194-Rats,
pubmed-meshheading:2123194-Restriction Mapping,
pubmed-meshheading:2123194-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2123194-Testosterone,
pubmed-meshheading:2123194-Vas Deferens
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pubmed:year |
1990
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pubmed:articleTitle |
Androgen-dependent protein from mouse vas deferens. cDNA cloning and protein homology with the aldo-keto reductase superfamily.
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pubmed:affiliation |
Physiologie Comparée et Endocrinologie, Centre National de la Recherche Scientifique U.A. 360, Université Blaise Pascal, Les Cézeaux, Aubière France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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