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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1990-12-28
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pubmed:abstractText |
Previous studies have demonstrated that 1-beta-D-arabinofuranosylcytosine (ara-C) induces terminal differentiation of human myeloid leukemia cells. Other studies have shown that the c-jun protooncogene is expressed during phorbol ester-induced myeloid differentiation. This work examines the effects of ara-C on c-jun gene expression in human KG-1 myeloid leukemia cells. The results demonstrate that c-jun transcripts are undetectable in uninduced KG-1 cells and that ara-C induces expression of this gene in a concentration- and time-dependent manner. Ara-C treatment was also associated with increases in c-jun transcripts in U-937, THP-1, and HL-60 myeloid leukemia cells. Furthermore, transcriptional run-on analysis has demonstrated that exposure to ara-C increases the rate of c-jun gene transcription. The results also demonstrate that while inhibition of protein synthesis superinduces c-jun mRNA levels in phorbol ester-treated KG-1 cells, cycloheximide had no effect on the induction of c-jun transcripts during ara-C treatment. Moreover, the half-life of c-jun transcripts in ara-C-treated KG-1 cells was 42 min. These findings suggest that the increase in c-jun mRNA observed during ara-C treatment is regulated by a transcriptional mechanism, and that c-jun may be involved in the induction of differentiation and regulation of gene expression by ara-C.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2105946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2419912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2444869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2457172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2477086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2498162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2498322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2506186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2537468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2542296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2667136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2673023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2725496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2799385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2825349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-2998035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3033666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3040261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3136397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3136398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3142689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3151183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3186736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3345522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3347253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3531806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-3554236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-4530318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-5530557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-5549896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-5754840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-6784928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-6816625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-6893015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-6942429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-6958361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-7138584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2122980-7410404
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:geneSymbol |
c-jun
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1517-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2122980-Actins,
pubmed-meshheading:2122980-Blotting, Northern,
pubmed-meshheading:2122980-Cell Cycle,
pubmed-meshheading:2122980-Cell Differentiation,
pubmed-meshheading:2122980-Cytarabine,
pubmed-meshheading:2122980-DNA-Binding Proteins,
pubmed-meshheading:2122980-Dactinomycin,
pubmed-meshheading:2122980-Gene Expression Regulation,
pubmed-meshheading:2122980-Humans,
pubmed-meshheading:2122980-Kinetics,
pubmed-meshheading:2122980-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:2122980-Proto-Oncogene Proteins,
pubmed-meshheading:2122980-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:2122980-Proto-Oncogenes,
pubmed-meshheading:2122980-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2122980-Transcription, Genetic,
pubmed-meshheading:2122980-Transcription Factors,
pubmed-meshheading:2122980-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Transcriptional regulation of c-jun gene expression by arabinofuranosylcytosine in human myeloid leukemia cells.
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pubmed:affiliation |
Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article
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