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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1990-11-15
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pubmed:abstractText |
With the commercial availability of the improved colorimetric assay for fructosamine, we established the fructosamine values for non-diabetic healthy persons. This improved assay incorporates detergent and uricase in a more concentrated nitroblue-tetrazolium reagent base compared to the earlier reagent (Johnson & Baker assay), including a new serum calibrator. An evaluation of the assay showed that fructosamine values obtained with serum, EDTA-plasma and heparin-plasma were comparable to each other. There was no apparent effect on fructosamine by lipids. Within-run precision variations for each specimen type (fructosamine 248-592 mumol/l), determined with a mechanised analyser were less than 3% (0.9-3%). Between-run precision for serum and EDTA-plasma ranged from 2.5% to 5.6%. This fructosamine assay also showed a positive correlation with the Johnson and Baker assay. Based on 48 healthy adults (20 males, 28 females) of mean age 32.9 +/- 9.2 years, the fructosamines ranged from 195 to 304 mumol/l, with a mean 250 +/- 24.3 mumol/l. The 2.5 and 97.5 percentiles were 196.4 mumol/l and 301.8 mumol/l respectively.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Fructosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Hexosamines,
http://linkedlifedata.com/resource/pubmed/chemical/Triglycerides
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0304-4602
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
480-3
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2121089-Adult,
pubmed-meshheading:2121089-Cholesterol,
pubmed-meshheading:2121089-Colorimetry,
pubmed-meshheading:2121089-Diabetes Mellitus,
pubmed-meshheading:2121089-Edetic Acid,
pubmed-meshheading:2121089-Female,
pubmed-meshheading:2121089-Fructosamine,
pubmed-meshheading:2121089-Heparin,
pubmed-meshheading:2121089-Hexosamines,
pubmed-meshheading:2121089-Humans,
pubmed-meshheading:2121089-Male,
pubmed-meshheading:2121089-Triglycerides
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pubmed:year |
1990
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pubmed:articleTitle |
An improved colorimetric assay for fructosamine.
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pubmed:affiliation |
Department of Medicine, National University of Singapore.
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pubmed:publicationType |
Journal Article,
Comparative Study
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