Linked Life Data

21207376

Source:http://linkedlifedata.com/resource/pubmed/id/21207376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-1-5
pubmed:abstractText
Adult mouse lung epithelial stem/progenitor cells (EpiSPC) can be defined in vitro as epithelial colony-forming units that are capable of self-renewal, and which when co-cultured with lung mesenchymal stromal cells (MSC) are able to give rise to differentiated progeny comprising mature lung epithelial cells. This unit describes a protocol for the prospective isolation and in vitro propagation and differentiation of adult mouse lung EpiSPC. The strategy used for selection of EpiSPC and MSC from adult mouse lung by enzymatic digestion and flow cytometry is based on the differential expression of CD45, CD31, Sca-1, EpCAM, and CD24. The culture conditions required for the differentiation (co-culture with MSC) and expansion (stromal-free culture with FGF-10 and HGF) of EpiSPC are described.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1938-8969
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 2G.1
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Isolation and clonal assay of adult lung epithelial stem/progenitor cells.
pubmed:affiliation
Lung Regeneration Laboratory, The Department of Pharmacology, University of Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article