Linked Life Data

2120354

Source:http://linkedlifedata.com/resource/pubmed/id/2120354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-11-6
pubmed:abstractText
To investigate whether Langerhans cells are capable of inducing contact sensitivity effector T cells, we incubated purified T cells from naive mice with syngeneic cultured trinitrophenyl-modified Langerhans cells for 4-5 d. The cells were then expanded in interleukin-2 and fresh medium for another 6-9 d and injected intravenously into naive syngeneic recipient mice. After the ears of recipient mice were painted with 1% trinitrochlorobenzene, we observed an ear-swelling response peaking at 24-48 h. The ear-swelling response was hapten specific. CD8- but not CD4-depleted T cells mediated strong contact sensitivity. Systemic adoptive transfer into nude mice also lead to a hapten-specific delayed ear-swelling response. However, this response was less protracted than in euthymic animals, suggesting the participation of the recipient (non-immunized) T cells in the ear-swelling response of the euthymic mice. Lymphokine analysis of in vitro primed and restimulated T cells revealed predominant production of interleukin-2 but little or no interleukin-4. These in vitro primed cells therefore resemble type 1 or inflammatory T helper cell clones.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-202X
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
436-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Cultured epidermal Langerhans cells activate effector T cells for contact sensitivity.
pubmed:affiliation
Department of Dermatology, Hôpital Cantonal Universitaire, Geneva, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't