Source:http://linkedlifedata.com/resource/pubmed/id/21198750
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2011-1-4
|
pubmed:abstractText |
Human CD26 has dipeptidyl peptidase-4 (DPP IV) enzyme activity and binds to adenosine deaminase (ADA). CD26 is costimulatory for lymphocytes and has a circulating soluble form (sCD26). DPP IV enzyme inhibition is a new successful type 2 diabetes therapy. We examined whether the ADA binding and catalytic functions of sCD26 contribute to its effects on T-cell proliferation. Wildtype soluble recombinant human CD26 (srhCD26), an enzyme inactive mutant (srhCD26E-) and an ADA non-binding mutant (srhCD26A-) were co-incubated in in vitro T-cell proliferation assays with peripheral blood mononuclear cells (PBMC) stimulated with phytohaemagglutinin (PHA), muromonab-CD3 or Herpes simplex virus antigen (HSV Ag). Both srhCD26 and srhCD26E- enhanced PHA-induced T-cell proliferation dose-dependently in all six subjects tested. srhCD26 and srhCD26A- had no overall effect on anti-CD3-stimulated PBMC proliferation in four of five subjects. srhCD26, srhCD26E- and srhCD26A- enhanced HSV Ag induced PBMC proliferation in low responders to HSV Ag, but had no effect or inhibited proliferation in HSV-high responders. Thus, effects of soluble human CD26 on human T-cell proliferation are mechanistically independent of both the enzyme activity and the ADA-binding capability of sCD26.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
1365-3083
|
pubmed:author | |
pubmed:copyrightInfo |
© 2011 The Authors. Scandinavian Journal of Immunology © 2011 Blackwell Publishing Ltd.
|
pubmed:issnType |
Electronic
|
pubmed:volume |
73
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
102-11
|
pubmed:meshHeading |
pubmed-meshheading:21198750-Adenosine Deaminase,
pubmed-meshheading:21198750-Adult,
pubmed-meshheading:21198750-Cell Proliferation,
pubmed-meshheading:21198750-Cells, Cultured,
pubmed-meshheading:21198750-Dipeptidyl Peptidase 4,
pubmed-meshheading:21198750-Female,
pubmed-meshheading:21198750-Humans,
pubmed-meshheading:21198750-Lymphocyte Activation,
pubmed-meshheading:21198750-Lymphocytes,
pubmed-meshheading:21198750-Male,
pubmed-meshheading:21198750-Middle Aged,
pubmed-meshheading:21198750-Protein Binding,
pubmed-meshheading:21198750-Solubility,
pubmed-meshheading:21198750-Young Adult
|
pubmed:year |
2011
|
pubmed:articleTitle |
Soluble CD26?/?dipeptidyl peptidase IV enhances human lymphocyte proliferation in vitro independent of dipeptidyl peptidase enzyme activity and adenosine deaminase binding.
|
pubmed:affiliation |
A.W. Morrow Gastroenterology and Liver Centre, Royal Prince Alfred Hospital, Centenary Institute and Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|