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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2011-4-15
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pubmed:abstractText |
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1477-2566
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
594-7
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pubmed:meshHeading |
pubmed-meshheading:21198335-Adipose Tissue,
pubmed-meshheading:21198335-Adult,
pubmed-meshheading:21198335-Animals,
pubmed-meshheading:21198335-Cell Count,
pubmed-meshheading:21198335-Cell Culture Techniques,
pubmed-meshheading:21198335-Cell Differentiation,
pubmed-meshheading:21198335-Cell Separation,
pubmed-meshheading:21198335-Cell Survival,
pubmed-meshheading:21198335-Cells, Cultured,
pubmed-meshheading:21198335-Culture Media,
pubmed-meshheading:21198335-Female,
pubmed-meshheading:21198335-Humans,
pubmed-meshheading:21198335-Immunophenotyping,
pubmed-meshheading:21198335-Mesenchymal Stem Cells,
pubmed-meshheading:21198335-Middle Aged,
pubmed-meshheading:21198335-Recombinant Proteins,
pubmed-meshheading:21198335-Stromal Cells,
pubmed-meshheading:21198335-Swine,
pubmed-meshheading:21198335-Trypsin,
pubmed-meshheading:21198335-Young Adult
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pubmed:year |
2011
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pubmed:articleTitle |
Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
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pubmed:affiliation |
Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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