Linked Life Data

21195574

Source:http://linkedlifedata.com/resource/pubmed/id/21195574

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-1-25
pubmed:abstractText
In this study, a sensitive and robust ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed, validated, and applied to determine gender-dependent pharmacokinetics of total emodin (aglycone+glucuronide) in male and female Sprague-Dawley rats. The lower limit of quantification for emodin and emodin glucuronide in rat plasma was 39 and 78 ng/ml, with signal-to-noise ratio of ? 10. Precision and accuracy studies showed emodin and emodin glucuronide plasma concentrations well within the 10% range in all studies. Plasma recovery of emodin and emodin glucuronide was always above 86% for low (emodin: 39 ng/ml; glucuronide: 78 ng/ml), 92% for medium (625 ng/ml), and 97% for high (10000 ng/ml) concentrations. Furthermore, emodin showed more than 95% plasma stability under short-term and long-term storage conditions, as well as after three freeze-thaw cycles in the experiments. The developed and validated analytical method was successfully applied to study the gender-dependent 10-fold higher oral bioavailability of total emodin in male than female rats. The oral bioavailability of emodin and emodin glucuronide was also measured separately and showed a statistically significant gender difference in oral bioavailability of emodin and emodin glucuronide in rats.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-264X
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1157-62
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Sensitive and robust UPLC-MS/MS method to determine the gender-dependent pharmacokinetics in rats of emodin and its glucuronide.
pubmed:affiliation
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural