Source:http://linkedlifedata.com/resource/pubmed/id/21193223
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2011-2-14
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| pubmed:abstractText |
Inhibitor of growth 5, a tumor suppressor protein, can interact with p53, thereby inhibiting cell growth and inducing apoptosis. Inhibitor of growth 5 overexpression results in a reduction in colony-forming efficiency and cell population in S phase. To clarify the roles of inhibitor of growth 5 in tumorigenesis and progression of colorectal carcinomas, we examined inhibitor of growth 5 expression by immunohistochemistry on a tissue microarray containing colorectal carcinomas (n = 306), adenomas (n = 69), and nonneoplastic mucosa (n = 288) and compared this with clinicopathologic parameters of the carcinomas. In addition, inhibitor of growth 5 expression in colorectal carcinoma tissues and cell lines (DLD-1, HCT-15, SW480, and WiDr) was analyzed by Western blot and reverse transcriptase-polymerase chain reaction. It was found that the inhibitor of growth 5 protein was localized to the nuclei of colon carcinoma cells with no differences at mRNA levels. Among 18 frozen samples of colorectal carcinoma, significantly increased expression of inhibitor of growth 5 protein was observed in the carcinoma in comparison with adjacent mucosa in 14 cases (77.8%; P < .05), and 71.4% (10/14) of carcinoma cases exhibited up-regulated inhibitor of growth 5 mRNA expression. Decreased inhibitor of growth 5 expression was detected by immunohistochemistry in colorectal carcinoma, compared with non-neoplastic mucosa and adenoma (P < .05). Nuclear inhibitor of growth 5 expression was negatively correlated with tumor size, depth of invasion, degree of dedifferentiation, and Union Internationale Contre le Cancer staging (P < .05). In contrast, cytoplasmic inhibitor of growth 5 expression was positively correlated with depth of invasion, lymphatic invasion, and Union Internationale Contre le Cancer staging (P < .05). It was suggested that aberrant inhibitor of growth 5 expression may contribute to pathogenesis, growth, and invasion of colorectal carcinomas and could be considered as a promising marker to gauge aggressiveness of colorectal carcinomas.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1532-8392
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| pubmed:author | |
| pubmed:copyrightInfo |
Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
42
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
424-33
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| pubmed:meshHeading |
pubmed-meshheading:21193223-Adenocarcinoma,
pubmed-meshheading:21193223-Adenoma,
pubmed-meshheading:21193223-Aged,
pubmed-meshheading:21193223-Blotting, Western,
pubmed-meshheading:21193223-Cell Line, Tumor,
pubmed-meshheading:21193223-Cell Nucleus,
pubmed-meshheading:21193223-Cell Transformation, Neoplastic,
pubmed-meshheading:21193223-Colorectal Neoplasms,
pubmed-meshheading:21193223-Cytoplasm,
pubmed-meshheading:21193223-Female,
pubmed-meshheading:21193223-Fluorescent Antibody Technique, Direct,
pubmed-meshheading:21193223-Humans,
pubmed-meshheading:21193223-Male,
pubmed-meshheading:21193223-Middle Aged,
pubmed-meshheading:21193223-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21193223-Tissue Array Analysis,
pubmed-meshheading:21193223-Transcription Factors,
pubmed-meshheading:21193223-Tumor Suppressor Proteins
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| pubmed:year |
2011
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| pubmed:articleTitle |
The nuclear to cytoplasmic shift of ING5 protein during colorectal carcinogenesis with their distinct links to pathologic behaviors of carcinomas.
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| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang 110001, China. zheng_huachuan@hotmail.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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