Linked Life Data

21192003

Source:http://linkedlifedata.com/resource/pubmed/id/21192003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-4-13
pubmed:abstractText
Matrix mineralization is a terminal process in osteoblast differentiation, and several approaches have been introduced to characterize the process in tissues or cultured cells. However, an analytical technique that quantitates in vitro matrix mineralization of live cells without any labeling or complex treatments is still lacking. In this study, we investigate a simple and enhanced optical method based on surface plasmon resonance (SPR) detection that can monitor the surface-limited refractive index change in real-time. During monitoring MC3T3-E1 cells in vitro culture every 2 days for over 4 weeks, the SPR angle is shifted with a greater resonance change in cells cultured with osteogenic reagents than those without the reagents. In addition, the SPR results obtained have a close relevance with the tendency of conventional mineralization staining and an inductively coupled plasma-based calcium content measure. These results suggest a new approach of a real-time SPR monitoring in vitro matrix mineralization of cultured cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1097-0290
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Wiley Periodicals, Inc.
pubmed:issnType
Electronic
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1473-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Preliminary approach of real-time monitoring in vitro matrix mineralization based on surface plasmon resonance detection.
pubmed:affiliation
School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-742, Korea.
pubmed:publicationType
Letter, Research Support, Non-U.S. Gov't