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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2011-2-9
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pubmed:abstractText |
The pancreatic zymogen granule membrane protein (GP2) is expressed by pancreatic acinar cells and M cells of the ileum. GP2 is the closest related homologue of the urine resident Tamm-Horsfall protein (THP). Recently, it was shown that THP is a ligand of various scavenger receptors (SRs). Therefore, we were interested, if GP2 has similar properties. cDNA of different SRs was stably transfected into a murine thymoma cell line. GP2 was recombinantly expressed, purified and biotinylated. Binding or uptake of GP2 by transfected cells or monocyte-derived dendritic cells (moDCs) was analyzed by flow-cytometry. GP2 is a binding partner of the scavenger receptor expressed on endothelial cells I (SREC-I) but not of SR-AI and SR-BI. The dissociation constant (K(d)) of GP2 binding to SREC-I is 41.3nM. SREC transfected cells are able to internalize GP2. moDCs express SREC-I and also bind and internalize GP2. Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding. Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response. In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21190681-10612411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21190681-10825295,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21190681-9586636
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1090-2163
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
88-93
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pubmed:dateRevised |
2011-9-15
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pubmed:meshHeading |
pubmed-meshheading:21190681-Animals,
pubmed-meshheading:21190681-Antibodies,
pubmed-meshheading:21190681-Cell Line, Tumor,
pubmed-meshheading:21190681-Dendritic Cells,
pubmed-meshheading:21190681-Endocytosis,
pubmed-meshheading:21190681-GPI-Linked Proteins,
pubmed-meshheading:21190681-Humans,
pubmed-meshheading:21190681-Mice,
pubmed-meshheading:21190681-Protein Binding,
pubmed-meshheading:21190681-Recombinant Proteins,
pubmed-meshheading:21190681-Scavenger Receptors, Class F,
pubmed-meshheading:21190681-T-Lymphocytes,
pubmed-meshheading:21190681-Transduction, Genetic
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pubmed:year |
2011
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pubmed:articleTitle |
The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).
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pubmed:affiliation |
Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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