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rdf:type |
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lifeskim:mentions |
umls-concept:C0002345,
umls-concept:C0007600,
umls-concept:C0009015,
umls-concept:C0017337,
umls-concept:C0025920,
umls-concept:C0205314,
umls-concept:C0220908,
umls-concept:C0591833,
umls-concept:C0679622,
umls-concept:C1257975,
umls-concept:C1880022
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pubmed:issue |
12
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pubmed:dateCreated |
2010-12-29
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pubmed:abstractText |
A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-?1 and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1976-670X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-94
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pubmed:meshHeading |
pubmed-meshheading:21189154-Alternative Splicing,
pubmed-meshheading:21189154-Animals,
pubmed-meshheading:21189154-Cloning, Molecular,
pubmed-meshheading:21189154-Gene Library,
pubmed-meshheading:21189154-Green Fluorescent Proteins,
pubmed-meshheading:21189154-Imidazoles,
pubmed-meshheading:21189154-Lac Operon,
pubmed-meshheading:21189154-Mesenchymal Stem Cells,
pubmed-meshheading:21189154-Mice,
pubmed-meshheading:21189154-Mice, Inbred C3H,
pubmed-meshheading:21189154-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:21189154-Nuclear Proteins,
pubmed-meshheading:21189154-Pyridines,
pubmed-meshheading:21189154-Transforming Growth Factor beta1
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pubmed:year |
2010
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pubmed:articleTitle |
Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening.
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pubmed:affiliation |
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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