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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2010-12-28
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pubmed:abstractText |
Human serum has the potential to become the most informative source of novel biomarkers, but its study is very difficult due to the incredible complexity of its molecular composition. We describe a novel tool based on biodegradable nanoporous nanoparticles (NPNPs) that allows the harvesting of low-molecular-weight fractions of crude human serum or other biofluids. NPNPs with a diameter of 200 nm and pore size of a few nm were obtained by ultrasonication of nanoporous silicon. When incubated with a solution, the NPNPs harvest only the molecules small enough to be absorbed into the nanopores. Then they can be recovered by centrifugation and dissolved in water, making the harvested molecules available for further analyses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1178-2013
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1005-15
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pubmed:meshHeading |
pubmed-meshheading:21187942-Biological Markers,
pubmed-meshheading:21187942-Blood Proteins,
pubmed-meshheading:21187942-Dextrans,
pubmed-meshheading:21187942-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:21187942-Filtration,
pubmed-meshheading:21187942-Fluorescein-5-isothiocyanate,
pubmed-meshheading:21187942-Humans,
pubmed-meshheading:21187942-Hydrogen-Ion Concentration,
pubmed-meshheading:21187942-Mass Spectrometry,
pubmed-meshheading:21187942-Microscopy, Electron, Scanning,
pubmed-meshheading:21187942-Microscopy, Fluorescence,
pubmed-meshheading:21187942-Molecular Weight,
pubmed-meshheading:21187942-Nanoparticles,
pubmed-meshheading:21187942-Nanopores,
pubmed-meshheading:21187942-Nanotechnology,
pubmed-meshheading:21187942-Porosity,
pubmed-meshheading:21187942-Silicon,
pubmed-meshheading:21187942-Solubility,
pubmed-meshheading:21187942-Temperature
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pubmed:year |
2010
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pubmed:articleTitle |
Highly efficient human serum filtration with water-soluble nanoporous nanoparticles.
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pubmed:affiliation |
BIONEM Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro "Magna Graecia", Germaneto (CZ), Italy.
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pubmed:publicationType |
Journal Article
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