|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4973
|
|
pubmed:dateCreated |
1990-10-10
|
|
pubmed:abstractText |
The proto-oncogenes c-fos and c-jun function cooperatively as inducible transcription factors in signal transduction processes. Their protein products, Fos and Jun, form a heterodimeric complex that interacts with the DNA regulatory element known as the activator protein-1 (AP-1) binding site. Dimerization occurs via interaction between leucine zipper domains and serves to bring into proper juxtaposition a region in each protein that is rich in basic amino acids and that forms a DNA-binding domain. DNA binding of the Fos-Jun heterodimer was modulated by reduction-oxidation (redox) of a single conserved cysteine residue in the DNA-binding domains of the two proteins. Furthermore, a nuclear protein was identified that reduced Fos and Jun and stimulated DNA-binding activity in vitro. These results suggest that transcriptional activity mediated by AP-1 binding factors may be regulated by a redox mechanism.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0036-8075
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
7
|
|
pubmed:volume |
249
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1157-61
|
|
pubmed:dateRevised |
2007-3-19
|
|
pubmed:meshHeading |
pubmed-meshheading:2118682-Amino Acid Sequence,
pubmed-meshheading:2118682-Animals,
pubmed-meshheading:2118682-Cell-Free System,
pubmed-meshheading:2118682-Cysteine,
pubmed-meshheading:2118682-DNA Mutational Analysis,
pubmed-meshheading:2118682-DNA-Binding Proteins,
pubmed-meshheading:2118682-Diamide,
pubmed-meshheading:2118682-Humans,
pubmed-meshheading:2118682-Molecular Sequence Data,
pubmed-meshheading:2118682-Oxidation-Reduction,
pubmed-meshheading:2118682-Proto-Oncogene Proteins,
pubmed-meshheading:2118682-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:2118682-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:2118682-Rats,
pubmed-meshheading:2118682-Recombinant Proteins,
pubmed-meshheading:2118682-Signal Transduction,
pubmed-meshheading:2118682-Structure-Activity Relationship,
pubmed-meshheading:2118682-Sulfhydryl Reagents,
pubmed-meshheading:2118682-Transcription Factors
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Redox regulation of fos and jun DNA-binding activity in vitro.
|
|
pubmed:affiliation |
Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, NJ 07110.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
