Source:http://linkedlifedata.com/resource/pubmed/id/21186059
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2011-1-18
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| pubmed:abstractText |
Diblock copolymers (PEI-PCL) of poly(?-caprolactone) (PCL) and linear poly(ethylene imine) (PEI) were synthesized and assembled to biodegradable nano-carriers for co-delivery of BCL-2 siRNA and doxorubicin (DOX). Folic acid as a tumor-targeting ligand was conjugated to the polyanion, poly(ethylene glycol)-block-poly(glutamic acid) (FA-PEG-PGA). Driven by the electrostatic interaction, FA-PEG-PGA was coated onto the surface of the cationic PEI-PCL nanoparticles pre-loaded with siRNA and DOX, potentiating a ligand-directed delivery to human hepatic cancer cells Bel-7402. At certain N/P and C/N ratios (N/P: PEI-PCL nitrogen to siRNA phosphate; C/N: FA-PEG-PGA carboxyl to PEI-PCL amine), the nanoparticles exhibited not only high transfection efficiency but also ideally controlled release of drug. Compared to non-specific delivery, the folate-targeted delivery of BCL-2 siRNA resulted in more significant gene suppression at both the BCL-2 mRNA and protein expression levels, inducing cancer cell apoptosis and improving the therapeutic efficacy of the co-administered DOX. Herein we demonstrated that co-loading siRNA and small molecular drug in a multifunctional hierarchical nano-assembly enabled simultaneously delivering siRNA and drug into the same cancer cells, yielding synergistic effect of RNA interference and chemotherapy in cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Cations,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Micelles,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1878-5905
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
32
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2222-32
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| pubmed:meshHeading |
pubmed-meshheading:21186059-Antineoplastic Agents,
pubmed-meshheading:21186059-Apoptosis,
pubmed-meshheading:21186059-Biocompatible Materials,
pubmed-meshheading:21186059-Cations,
pubmed-meshheading:21186059-Cell Line,
pubmed-meshheading:21186059-Doxorubicin,
pubmed-meshheading:21186059-Drug Carriers,
pubmed-meshheading:21186059-Drug Delivery Systems,
pubmed-meshheading:21186059-Humans,
pubmed-meshheading:21186059-Liver Neoplasms,
pubmed-meshheading:21186059-Materials Testing,
pubmed-meshheading:21186059-Micelles,
pubmed-meshheading:21186059-Nanostructures,
pubmed-meshheading:21186059-Particle Size,
pubmed-meshheading:21186059-Polyethylene Glycols,
pubmed-meshheading:21186059-Polymers,
pubmed-meshheading:21186059-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:21186059-RNA, Small Interfering
|
| pubmed:year |
2011
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| pubmed:articleTitle |
The synergistic effect of hierarchical assemblies of siRNA and chemotherapeutic drugs co-delivered into hepatic cancer cells.
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| pubmed:affiliation |
Center of Biomedical Engineering, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510275, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|