Linked Life Data

2118547

Source:http://linkedlifedata.com/resource/pubmed/id/2118547

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-10-9
pubmed:abstractText
Sperm from 10 fertile donors were incubated for 8 h in a capacitating medium (BWW 3.5% HSA) and treated with 1.25 microliters of 2 mM Ca-ionophore solution. Sperm motion was analysed using the Hamilton-Thorn system before and after incubation and treatment. The acrosome reaction was detected with PSA-FITC labelling of the acrosome. Critical values of sperm movement parameters were defined for objective identification of hyperactive movement by the Hamilton-Thorn system. The incidence of hyperactive movement in the sperm suspensions was approximately 2%. No significant changes of hyperactive movement could be observed after the incubation period or the ionophore treatment, in contrast to a significant rise in the percentage of acrosome-reacted cells after ionophore treatment. The implications of these findings for monitoring hyperactive cells to test sperm function are discussed.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
593-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Objective identification of hyperactivated human spermatozoa by computerized sperm motion analysis with the Hamilton-Thorn sperm motility analyser.
pubmed:affiliation
Institute of Reproductive Medicine of the University, Münster, FRG.
pubmed:publicationType
Journal Article