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pubmed-article:21184801pubmed:abstractTextAutologous bone marrow (BM) cells with a faulty gene corrected by gene targeting could provide a powerful therapeutic option for patients with genetic blood diseases. Achieving this goal is hindered by the low abundance of therapeutically useful BM cells and the difficulty maintaining them in tissue culture long enough to complete gene targeting without differentiating. Our objective was to devise a simple long-term culture system, using unfractioned BM cells, that maintains and expands therapeutically useful cells for ?4 weeks.lld:pubmed
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pubmed-article:21184801pubmed:copyrightInfoPublished by Elsevier Inc.lld:pubmed
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pubmed-article:21184801pubmed:pagination375-83, 383.e1-4lld:pubmed
pubmed-article:21184801pubmed:dateRevised2011-9-26lld:pubmed
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pubmed-article:21184801pubmed:articleTitleTherapeutic benefits in thalassemic mice transplanted with long-term-cultured bone marrow cells.lld:pubmed
pubmed-article:21184801pubmed:affiliationDepartment of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, NC, USA. seigo.hatada@cshs.orglld:pubmed
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