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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2011-2-28
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pubmed:abstractText |
Autologous bone marrow (BM) cells with a faulty gene corrected by gene targeting could provide a powerful therapeutic option for patients with genetic blood diseases. Achieving this goal is hindered by the low abundance of therapeutically useful BM cells and the difficulty maintaining them in tissue culture long enough to complete gene targeting without differentiating. Our objective was to devise a simple long-term culture system, using unfractioned BM cells, that maintains and expands therapeutically useful cells for ?4 weeks.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1873-2399
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pubmed:author |
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pubmed:copyrightInfo |
Published by Elsevier Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
375-83, 383.e1-4
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:21184801-Animals,
pubmed-meshheading:21184801-Bone Marrow Cells,
pubmed-meshheading:21184801-Bone Marrow Transplantation,
pubmed-meshheading:21184801-Cell Culture Techniques,
pubmed-meshheading:21184801-Cells, Cultured,
pubmed-meshheading:21184801-Coculture Techniques,
pubmed-meshheading:21184801-Female,
pubmed-meshheading:21184801-Gene Expression Regulation,
pubmed-meshheading:21184801-Male,
pubmed-meshheading:21184801-Mice,
pubmed-meshheading:21184801-Mice, Transgenic,
pubmed-meshheading:21184801-Thalassemia,
pubmed-meshheading:21184801-Time Factors,
pubmed-meshheading:21184801-Transplantation, Autologous
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pubmed:year |
2011
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pubmed:articleTitle |
Therapeutic benefits in thalassemic mice transplanted with long-term-cultured bone marrow cells.
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pubmed:affiliation |
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, NC, USA. seigo.hatada@cshs.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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