Linked Life Data

21182692

Source:http://linkedlifedata.com/resource/pubmed/id/21182692

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-12-24
pubmed:abstractText
Bacterial biofilms have been observed and reported on food and food-processing surfaces and can contribute to increased risks for product quality and food safety. The colonization of fruit and vegetables by pectynolitic bacteria like Pseudonomas fluorescens attributable to conditions such as soft rot, can also manifest as biofilms. A developed biofilm structure can provide a protective environment for pathogens such as Listeria monocytogenes reducing the effectiveness of sanitisers and other inhibitory agents. Understanding the colonization of bacteria on leaf surfaces is essential to the development of a better understanding of the leaf ecology of vegetable products. Studies of microbial colonization of leaf surfaces have been conducted using SEM and more recently using confocal microsocpy techniques. In the current study, a Leica TCS NT laser scanning confocal microscope was used to investigate biofilm formation using vital fluorescence staining on intact vegetable leaves. Reflection contrast and fluorescence three-dimensional imaging successfully delineated bacterial and biofilm morphology without disturbing the bacterial or leaf surface structure. The results demonstrate the presence and development of biofilm on the surface of lettuce. The biofilms appeared to originate on the cuticle in distinct micro-environments such as in the natural depression of the stomata, or in the intercellular junction. Bacteria also adhered to and developed biofilm colonies within an hour of contact and with clean stainless steel surfaces. Our study investigates the progression of biofilm formation from leaf colonization, and will assist in characterising the critical mechanisms of plant/host interaction and facilitate the development of improved preservation, sanitising and packaging strategies for minimally processed vegetable products.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1365-2672
pubmed:author
pubmed:copyrightInfo
1998 Society of Applied Microbiology.
pubmed:issnType
Electronic
pubmed:volume
85 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45S-51S
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Bacterial colonization and biofilm development on minimally processed vegetables.
pubmed:affiliation
Institute of Land and Food Resources, The University of Melbourne, Parkville, VIC, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't