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pubmed-article:2118199pubmed:abstractTextCalcium-dependent and -independent Sepharose 4B-binding bovine serum proteins were isolated by affinity chromatography on unsubstituted Sepharose 4B using 2 mM ethylenediamine tetraacetic acid followed by 0.1 M galactose. They appeared in two different protein peaks by gel filtration on Sephacryl S-200. The earlier eluted protein was demonstrated to be immunologically IgM, whereas the retarded one IgG. From these findings, Sepharose 4B-binding bovine serum proteins are suggested to be calcium-dependent and -independent immunoglobulins IgM and IgG.lld:pubmed
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pubmed-article:2118199pubmed:articleTitleIdentification and isolation of calcium-dependent and -independent bovine immunoglobulins reactive with sepharose 4B.lld:pubmed
pubmed-article:2118199pubmed:affiliationDepartment of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.lld:pubmed
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