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rdf:type |
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lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0020792,
umls-concept:C0021027,
umls-concept:C0036682,
umls-concept:C0085862,
umls-concept:C0204727,
umls-concept:C0205332,
umls-concept:C0205409,
umls-concept:C1299583,
umls-concept:C1549571,
umls-concept:C1608386
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pubmed:issue |
4
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|
pubmed:dateCreated |
1990-10-4
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|
pubmed:abstractText |
Calcium-dependent and -independent Sepharose 4B-binding bovine serum proteins were isolated by affinity chromatography on unsubstituted Sepharose 4B using 2 mM ethylenediamine tetraacetic acid followed by 0.1 M galactose. They appeared in two different protein peaks by gel filtration on Sephacryl S-200. The earlier eluted protein was demonstrated to be immunologically IgM, whereas the retarded one IgG. From these findings, Sepharose 4B-binding bovine serum proteins are suggested to be calcium-dependent and -independent immunoglobulins IgM and IgG.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-5295
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
727-33
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pubmed:dateRevised |
2011-7-25
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pubmed:meshHeading |
pubmed-meshheading:2118199-Animals,
pubmed-meshheading:2118199-Blood Proteins,
pubmed-meshheading:2118199-Calcium-Binding Proteins,
pubmed-meshheading:2118199-Cattle,
pubmed-meshheading:2118199-Chromatography, Affinity,
pubmed-meshheading:2118199-Edetic Acid,
pubmed-meshheading:2118199-Hemagglutination,
pubmed-meshheading:2118199-Hemagglutination Inhibition Tests,
pubmed-meshheading:2118199-Immunodiffusion,
pubmed-meshheading:2118199-Immunoelectrophoresis,
pubmed-meshheading:2118199-Immunoglobulin G,
pubmed-meshheading:2118199-Immunoglobulin M,
pubmed-meshheading:2118199-Molecular Weight,
pubmed-meshheading:2118199-Protein Binding,
pubmed-meshheading:2118199-Sepharose
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|
pubmed:year |
1990
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|
pubmed:articleTitle |
Identification and isolation of calcium-dependent and -independent bovine immunoglobulins reactive with sepharose 4B.
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pubmed:affiliation |
Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
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pubmed:publicationType |
Journal Article
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