Source:http://linkedlifedata.com/resource/pubmed/id/21181914
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2010-12-24
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pubmed:abstractText |
During recent years, vaccination against hepatitis A has been implemented in several countries. It is expected that the increase in mass vaccination against hepatitis A will eventually result in a decreased prevalence of anti-HAV antibodies in the general population. For this reason, a suitable clinical sample for diagnosis of hepatitis A must be sufficiently sensitive to enable detection of lower antibodies titers. In this study, the feasibility of using dried blood spots (DBS) was assessed for the detection of anti-HAV antibodies after a natural infection and vaccination. Seventy-four DBS and paired plasma samples were obtained from a group of college students for a cross-sectional hepatitis A seroepidemiological study. Forty-six students seronegative for anti-HAV were selected randomly and immunized with an inactivated hepatitis A vaccine using an 0-6 month schedule. Seroconversion was monitored in paired plasma and DBS samples 6 months after the first dose followed by a period of 8 and 24 months after the second dose. A strong correlation between OD/CO rates of paired plasma and DBS samples for the detection of anti-HAV was observed. The sensitivity and specificity of the DBS compared with plasma for the detection of anti-HAV antibodies after natural infection was 100%. The sensitivity of DBS in samples collected 24 months after the second dose of hepatitis A vaccine was 95.4%. The results showed that DBS samples can be used for the detection of anti-HAV antibodies both after natural infection or vaccination.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1096-9071
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pubmed:author | |
pubmed:copyrightInfo |
2010 Wiley-Liss, Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
208-17
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pubmed:meshHeading |
pubmed-meshheading:21181914-Brazil,
pubmed-meshheading:21181914-Female,
pubmed-meshheading:21181914-Hepatitis A,
pubmed-meshheading:21181914-Hepatitis A Antibodies,
pubmed-meshheading:21181914-Hepatitis A Vaccines,
pubmed-meshheading:21181914-Hepatitis A Virus, Human,
pubmed-meshheading:21181914-Humans,
pubmed-meshheading:21181914-Immunoenzyme Techniques,
pubmed-meshheading:21181914-Male,
pubmed-meshheading:21181914-Sensitivity and Specificity,
pubmed-meshheading:21181914-Seroepidemiologic Studies,
pubmed-meshheading:21181914-Specimen Handling,
pubmed-meshheading:21181914-Vaccination,
pubmed-meshheading:21181914-Young Adult
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pubmed:year |
2011
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pubmed:articleTitle |
The use of dried blood spots for assessing antibody response to hepatitis A virus after natural infection and vaccination.
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pubmed:affiliation |
Department of Microbiology and Parasitology-Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
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pubmed:publicationType |
Journal Article,
Randomized Controlled Trial,
Research Support, Non-U.S. Gov't
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