Linked Life Data

21181115

Source:http://linkedlifedata.com/resource/pubmed/id/21181115

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-26
pubmed:abstractText
We have shown that ischaemic preconditioning ameliorates both the local periosteal and the systemic leukocyte activation evoked by limb ischaemia-reperfusion. We hypothesized that the activation of chemosensitive afferent nerves by transient ischaemia contributes to the protective mechanisms of ischaemic preconditioning via a calcitonin gene-related peptide (CGRP)-dependent mechanism. In Sprague-Dawley rats, 60-min complete limb ischaemia was followed by 180 min of reperfusion. In further experiments, the CGRP analogue hCGRP (0.3 ?g kg(-1)) or ischaemic preconditioning (2?×?10-min ischaemia/10-min reperfusion) was applied prior to the ischaemia-reperfusion insult. Ischaemic preconditioning was performed in three subgroups in which animals received the CGRP receptor antagonist CGRP(8-37) (30 ?g kg(-1) h(-1)), the chemosensitive afferent nerve inactivator resiniferatoxin (3?×?15 ?g kg(-1), sc), or vehicle. The effects of CGRP(8-37) and resiniferatoxin on ischaemia-reperfusion without ischaemic preconditioning were also evaluated. In the tibial periosteum of rats, intravital fluorescence microscopy and immunohistochemistry revealed significant attenuations of ischaemia-reperfusion-induced post-ischaemic leukocyte-endothelial interactions (rolling and adherence in the postcapillary venules) and tissue intracellular adhesion molecule expression following ischaemic preconditioning or hCGRP administration. Administration of CGRP(8-37) or pretreatment of animals with resiniferatoxin reversed the anti-inflammatory effects of limb ischaemic preconditioning, but failed to affect the microcirculatory consequences of ischaemia-reperfusion without ischaemic preconditioning. The results suggest that activation of the chemo- (capsaicin-) sensitive afferent nerves is involved in the mechanisms of microcirculatory anti-inflammatory protection provided by limb ischaemic preconditioning. Controlled activation of chemosensitive C-fibres or the CGRP receptors by the induction of ischaemic preconditioning or other means may furnish therapeutic benefit by ameliorating the periosteal microcirculatory consequences of tourniquet ischaemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1432-1912
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
383
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-89
pubmed:meshHeading
pubmed-meshheading:21181115-Animals, pubmed-meshheading:21181115-Calcitonin Gene-Related Peptide, pubmed-meshheading:21181115-Cell Adhesion, pubmed-meshheading:21181115-Diterpenes, pubmed-meshheading:21181115-Hindlimb, pubmed-meshheading:21181115-Humans, pubmed-meshheading:21181115-Intercellular Adhesion Molecule-1, pubmed-meshheading:21181115-Ischemic Preconditioning, pubmed-meshheading:21181115-Leukocyte Rolling, pubmed-meshheading:21181115-Leukocytes, pubmed-meshheading:21181115-Male, pubmed-meshheading:21181115-Microcirculation, pubmed-meshheading:21181115-Microscopy, Video, pubmed-meshheading:21181115-Nerve Fibers, pubmed-meshheading:21181115-Neurons, Afferent, pubmed-meshheading:21181115-Peptide Fragments, pubmed-meshheading:21181115-Periosteum, pubmed-meshheading:21181115-Rats, pubmed-meshheading:21181115-Rats, Sprague-Dawley, pubmed-meshheading:21181115-Receptors, Calcitonin Gene-Related Peptide, pubmed-meshheading:21181115-Reperfusion Injury, pubmed-meshheading:21181115-Sensory Receptor Cells, pubmed-meshheading:21181115-Venules
pubmed:year
2011
pubmed:articleTitle
Anti-inflammatory effects of limb ischaemic preconditioning are mediated by sensory nerve activation in rats.
pubmed:affiliation
Institute of Surgical Research, University of Szeged, Pécsi utca 6, 6720, Szeged, Hungary.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't