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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-9-24
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pubmed:abstractText |
C6 is a potentially therapeutic murine monoclonal antibody that recognizes the class 1 outer membrane protein of Neisseria meningitidis. C6 specifically immunoblots this antigen and augments in vitro killing of N. meningitidis bacteria. We describe a general method of obtaining the heavy and light chain variable-region sequence from immunoglobulin-secreting cells. The method uses mixed polymerase chain reaction (PCR) primers designed from the 5' end of the framework 1 (FR1) sequences of the heavy and light chains, and 3'-end primers for constant-region conserved sequences. The method has been applied to the cloning and sequencing of the variable region of C6 to construct a humanized monoclonal antibody. Rapid amplification and sequencing of variable regions by this general method have multiple applications in the study of the immune response to infectious diseases.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
121-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2117775-Amino Acid Sequence,
pubmed-meshheading:2117775-Animals,
pubmed-meshheading:2117775-Antibodies, Bacterial,
pubmed-meshheading:2117775-Antibodies, Monoclonal,
pubmed-meshheading:2117775-Bacterial Outer Membrane Proteins,
pubmed-meshheading:2117775-Base Sequence,
pubmed-meshheading:2117775-DNA, Bacterial,
pubmed-meshheading:2117775-Gene Amplification,
pubmed-meshheading:2117775-Immunoblotting,
pubmed-meshheading:2117775-Immunoglobulin Heavy Chains,
pubmed-meshheading:2117775-Immunoglobulin Light Chains,
pubmed-meshheading:2117775-Immunoglobulin Variable Region,
pubmed-meshheading:2117775-Mice,
pubmed-meshheading:2117775-Molecular Sequence Data,
pubmed-meshheading:2117775-Neisseria meningitidis,
pubmed-meshheading:2117775-Polymerase Chain Reaction,
pubmed-meshheading:2117775-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Immunoglobulin V regions of a bactericidal anti-Neisseria meningitidis outer membrane protein monoclonal antibody.
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pubmed:affiliation |
Genelabs Inc., Redwood City, California.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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