Linked Life Data

21176354

Source:http://linkedlifedata.com/resource/pubmed/id/21176354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-12-23
pubmed:abstractText
Interleukin-3 receptor (IL-3R) is a heterodimeric membrane receptor. The ? subunit is essential for ligand binding and confers ligand specificity to the receptor. The common beta chain (?c) subunit, which is shared by the granulocyte macrophage-colony stimulating factor (GM-CSF), IL-3 and IL-5 receptors, is required for high-affinity ligand binding and signal transduction, mediating growth and survival of hematopoietic progenitor cells and the production and activation of mature hematopoietic cells. In order to investigate the role of IL-3 receptor system (IL-3R?, GM-CSFR? and h?c) in myeloid differentiation, the expression level of IL-3 receptor system gene in all-trans retinoic acid (ATRA)-induced NB4 cell differentiation was detected by quantitative real time RT-PCR. At the same time, DNA sequence change was analyzed by cDNA sequencing. The results showed that the expression level of IL-3R? mRNA was obviously down-regulated in NB4 cells treated with ATRA for 24 hours, but during differentiation of ATRA induced NB4 cells, the expression level of IL-3R? mRNA was gradually restored, while the expression levels of GM-CSFR? mRNA and h?c mRNA were gradually up-regulated. The sequence of IL-3R? and GM-CSFR? gene did not change before and after NB4 cells differentiation, but the sequence of h?c gene changed when NB4 cells were treated with ATRA, the expression of h?c mRNA sequence before NB4 cell differentiation taken truncated mutation as dominant, as regards expression of h?c mRNA sequence after NB4 cell differentiation, the truncated mutation of h?c mRNA had restored to wild type. It is concluded that the IL-3 receptor abnormality exists in NB4 cells, over expression of IL-3R? and truncated mutation of h?c may be involved in proliferation and differentiation block in NB4 cells.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1009-2137
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1474-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
[Expression change of IL-3 receptor system in all-trans retinoic acid induced differentiation of NB4 cells].
pubmed:affiliation
Fujian Institute of Hematology, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't