Linked Life Data

21174402

Source:http://linkedlifedata.com/resource/pubmed/id/21174402

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-19
pubmed:abstractText
The alkyne-azide Cu(I)-catalyzed Huisgen cycloaddition, a click-type reaction, was used to label a double-stranded oligonucleotide (siRNA) with fluorine-18. An alkyne solid support CPG for the preparation of monostranded oligonucleotides functionalized with alkyne has been developed. Two complementary azide labeling agents (1-(azidomethyl)-4-[(18)F]fluorobenzene) and 1-azido-4-(3-[(18)F]fluoropropoxy)benzene have been produced with 41% and 35% radiochemical yields (decay-corrected), respectively. After annealing with the complementary strand, the siRNA was directly labeled by click chemistry with [(18)F]fluoroazide to produce the [(18)F]-radiolabeled siRNA with excellent radiochemical yield and purity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1520-4812
pubmed:author
pubmed:issnType
Electronic
pubmed:day
19
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
108-14
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
General method for labeling siRNA by click chemistry with fluorine-18 for the purpose of PET imaging.
pubmed:affiliation
Cyclotron Research Center, Universite? de Lie?ge, Sart-Tilman B.30, B-4000 Lie?ge, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't