Source:http://linkedlifedata.com/resource/pubmed/id/21169548
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-1-6
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| pubmed:abstractText |
The ectodomain of matrix protein 2 (M2e) of influenza A virus is an attractive target for a universal influenza A vaccine: the M2e sequence is highly conserved across influenza virus subtypes, and induced humoral anti-M2e immunity protects against a lethal influenza virus challenge in animal models. Clinical phase I studies with M2e vaccine candidates have been completed. However, the in vivo mechanism of immune protection induced by M2e-carrier vaccination is unclear. Using passive immunization experiments in wild-type, FcR?(-/-), Fc?RI(-/-), Fc?RIII(-/-), and (Fc?RI, Fc?RIII)(-/-) mice, we report in this study that Fc receptors are essential for anti-M2e IgG-mediated immune protection. M2e-specific IgG1 isotype Abs are shown to require functional Fc?RIII for in vivo immune protection but other anti-M2e IgG isotypes can rescue Fc?RIII(-/-) mice from a lethal challenge. Using a conditional cell depletion protocol, we also demonstrate that alveolar macrophages (AM) play a crucial role in humoral M2e-specific immune protection. Additionally, we show that adoptive transfer of wild-type AM into (Fc?RI, Fc?RIII)(-/-) mice restores protection by passively transferred anti-M2e IgG. We conclude that AM and Fc receptor-dependent elimination of influenza A virus-infected cells are essential for protection by anti-M2e IgG.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fcgr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Fcgr3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/M2 protein, Influenza A virus,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1550-6606
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
15
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| pubmed:volume |
186
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1022-31
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| pubmed:meshHeading |
pubmed-meshheading:21169548-Animals,
pubmed-meshheading:21169548-Cell Death,
pubmed-meshheading:21169548-Cytotoxicity, Immunologic,
pubmed-meshheading:21169548-Disease Models, Animal,
pubmed-meshheading:21169548-Female,
pubmed-meshheading:21169548-Immunization, Passive,
pubmed-meshheading:21169548-Immunoglobulin G,
pubmed-meshheading:21169548-Influenza A virus,
pubmed-meshheading:21169548-Influenza Vaccines,
pubmed-meshheading:21169548-Lymphocyte Depletion,
pubmed-meshheading:21169548-Macrophages, Alveolar,
pubmed-meshheading:21169548-Mice,
pubmed-meshheading:21169548-Mice, Inbred BALB C,
pubmed-meshheading:21169548-Mice, Inbred C57BL,
pubmed-meshheading:21169548-Mice, Knockout,
pubmed-meshheading:21169548-Orthomyxoviridae Infections,
pubmed-meshheading:21169548-Protein Interaction Domains and Motifs,
pubmed-meshheading:21169548-Receptors, Fc,
pubmed-meshheading:21169548-Receptors, IgG,
pubmed-meshheading:21169548-Viral Matrix Proteins
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| pubmed:year |
2011
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| pubmed:articleTitle |
Universal vaccine based on ectodomain of matrix protein 2 of influenza A: Fc receptors and alveolar macrophages mediate protection.
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| pubmed:affiliation |
Department for Molecular Biomedical Research, Flanders Institute of Biotechnology (VIB), B-9052 Ghent, Belgium.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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