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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-9-10
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pubmed:abstractText |
Normodense human eosinophils have been labeled in 1-0-alkyl-phosphatidylcholine (alkyl-PC) with 32P by incubating isolated cells with alkyl-[32P]lysoPC. Stimulation of these 32P-labeled cells with C5a, A23187 or PMA in the presence of 0.5% ethanol resulted in time- and dose-dependent formation of alkyl-[32P]phosphatidic acid (alkyl-[32P]PA) and alkyl-[32P]phosphatidylethanol (alkyl-[32P]PEt). Because cellular ATP does not contain 32P, alkyl-[32P]PA must have been formed by the hydrolytic action of phospholipase D (PLD) and not by the combined actions of phospholipase C and DG kinase. Regardless of the stimulating agent, alkyl-[32P]PEt formation paralleled that of alkyl-[32P]PA, suggesting that alkyl-PEt was the result of a PLD-catalyzed transphosphatidylation reaction between alkyl-PC and ethanol. These data provide the first definitive proof of receptor- and nonreceptor-mediated activation of PLD in normodense eosinophils derived from human blood.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
540-7
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:2116791-Calcimycin,
pubmed-meshheading:2116791-Complement C5a,
pubmed-meshheading:2116791-Dose-Response Relationship, Drug,
pubmed-meshheading:2116791-Enzyme Activation,
pubmed-meshheading:2116791-Eosinophils,
pubmed-meshheading:2116791-Ethanol,
pubmed-meshheading:2116791-Glycerophospholipids,
pubmed-meshheading:2116791-Humans,
pubmed-meshheading:2116791-Kinetics,
pubmed-meshheading:2116791-Lymphocyte Activation,
pubmed-meshheading:2116791-Phosphatidic Acids,
pubmed-meshheading:2116791-Phospholipase D,
pubmed-meshheading:2116791-Phospholipases,
pubmed-meshheading:2116791-Tetradecanoylphorbol Acetate
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pubmed:year |
1990
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pubmed:articleTitle |
Activation of phospholipase D in normodense human eosinophils.
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pubmed:affiliation |
Department of Allergy and Immunology, Schering-Plough Research, Bloomfield, NJ 07003.
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pubmed:publicationType |
Journal Article
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