Linked Life Data

21164669

Source:http://linkedlifedata.com/resource/pubmed/id/21164669

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2010-12-17
pubmed:abstractText
The feasibility of identifying cancer cells by measuring the refractive index (RI) distribution across a single live cell with ultrahigh resolution full-field optical coherence microscopy (FF-OCM) is presented. The FF-OCM is utilized to quantify integral RI distributions of unmodified cells without any cell treatments and used as a biophysical indicator for diagnosing cell malignancy. Firstly, the physical thickness distribution of the cell adherent to a culture dish is measured by taking a series of 0.6 µm resolved en-face tomograms. Subsequently, from the en-face image of the bottom surface of the cell or the top surface of the dish, the phase gain image of the cell is extracted. Then, from these two measurements the axially averaged RI map of the cell is extracted. The implemented FF-OCM system had a 0.8 µm axial resolution and the phase measurement sensitivity of the system was around 124 mrad. With the system, RI maps of several living cell lines of normal and cancer cells were constructed and quantitatively analyzed. The experiments showed that cancer cells had higher RI than normal ones. This approach using the FF-OCM has significant potential for cancer diagnosis and dynamic cell analysis as in situ label-free biophysical assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1094-4087
pubmed:author
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23285-95
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Full-field optical coherence microscopy for identifying live cancer cells by quantitative measurement of refractive index distribution.
pubmed:affiliation
Department of Information and Communications, Gwangju Institute of Science and Technology, 261 Chemdan-gwagiro, Buk-Gu, Gwangju 500-712, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't