Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-9-12
pubmed:abstractText
A 4-month trial involving 2,563 routine clinical specimens was conducted to compare the improved BACTEC TB system (12B medium) with the conventional Lowenstein-Jensen (LJ) media for the isolation, identification, and susceptibility testing of mycobacteria. One hundred sixty-two mycobacterial isolates were recovered, 147 (91%) with BACTEC and 118 (73%) with LJ media. Of these, 62 were Mycobacterium tuberculosis complex strains, 59 (95%) of which were isolated with BACTEC and 54 (87%) of which were isolated with LJ media. Of the remaining 100 isolates, which were mycobacteria other than tuberculosis (MOTT), BACTEC and LJ media detected 88 and 64%, respectively. The contamination rate was significantly higher in BACTEC (5%) than in LJ media (3.3%). The mean isolation time for M. tuberculosis complex with BACTEC was 15.5 days, compared with 25.6 days with LJ. For MOTT, the mean isolation times were 5.8 and 21.4 days, respectively. Identification of 32 M. tuberculosis complex isolates and 38 isolates of MOTT by the BACTEC NAP (p-nitro-alpha-acetylamino-beta-hydroxypropiophenone) inhibition test gave 100% agreement with conventional biochemical identifications. The results of susceptibility testing of 18 M. tuberculosis complex isolates with BACTEC agreed completely with those obtained by the resistance ratio method.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-122228, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-14102034, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-3296098, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-3932460, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-4338078, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-6194175, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-6195181, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-6422739, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-6429191, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-6435489, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-6787076, http://linkedlifedata.com/resource/pubmed/commentcorrection/2116446-96930
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1288-91
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens.
pubmed:affiliation
Division of Clinical Microbiology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
pubmed:publicationType
Journal Article, Comparative Study