Source:http://linkedlifedata.com/resource/pubmed/id/21155826
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2011-2-3
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| pubmed:abstractText |
• A mitogen-activated protein kinase kinase kinase (MAPKKK) double mutant, Arabidopsis homologue of nucleus and phragmoplast associated kinase (anp) anp2anp3, and the mitogen-activated protein kinase (MAPK) 4 mutant mpk4 of Arabidopsis thaliana show prominent cytokinetic defects. This prompted the analysis of mitotic and cytokinetic progression as a function of MAPK signalling. Mutants were compared with wild types untreated or treated with the specific MAPKK inhibitor PD98059. • This study included phenotype analysis, expression analysis of the MPK4 promoter, immunofluorescent localization of MPK4, tubulin and MAP65-1, and time-lapse microscopic visualization of the mitotic microtubule (MT) transitions in control, mutant and inhibitor-treated cells. • Mutant and inhibitor-treated cells showed defects in mitosis and cytokinesis, including aberrant spindle and phragmoplast formation and drastically delayed or abortive mitosis and cytokinesis. As a result, bi- and multinucleate cells were formed, ultimately disturbing the vegetative tissue patterning. MPK4 was localized to all stages of the expanding phragmoplast, in a pattern similar to that of its putative substrate MAP65-1. • In this study, MPK4 is shown to be involved in the regulation of mitosis/cytokinesis through modulation of the cell division plane and cytokinetic progression.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/AtMPK4 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/MAP65-1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1469-8137
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| pubmed:author | |
| pubmed:copyrightInfo |
© 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
189
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1069-83
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| pubmed:meshHeading |
pubmed-meshheading:21155826-Arabidopsis,
pubmed-meshheading:21155826-Arabidopsis Proteins,
pubmed-meshheading:21155826-Cell Nucleus,
pubmed-meshheading:21155826-Cell Survival,
pubmed-meshheading:21155826-Cytokinesis,
pubmed-meshheading:21155826-Flavonoids,
pubmed-meshheading:21155826-Fluorescent Antibody Technique,
pubmed-meshheading:21155826-Imaging, Three-Dimensional,
pubmed-meshheading:21155826-Meristem,
pubmed-meshheading:21155826-Microscopy, Fluorescence,
pubmed-meshheading:21155826-Microtubule-Associated Proteins,
pubmed-meshheading:21155826-Microtubules,
pubmed-meshheading:21155826-Mitogen-Activated Protein Kinases,
pubmed-meshheading:21155826-Mitosis,
pubmed-meshheading:21155826-Mitotic Spindle Apparatus,
pubmed-meshheading:21155826-Mutation,
pubmed-meshheading:21155826-Phenotype,
pubmed-meshheading:21155826-Protein Kinase Inhibitors
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| pubmed:year |
2011
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| pubmed:articleTitle |
Mitogen-activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana.
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| pubmed:affiliation |
Institut für Zelluläre und Molekulare Botanik, Universität Bonn, Kirschallee 1, D53115 Bonn, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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