Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-1-28
pubmed:abstractText
The predatory bacterium Bdellovibrio bacteriovorus uses flagellar motility to locate regions rich in Gram-negative prey bacteria, colliding and attaching to prey and then ceasing flagellar motility. Prey are then invaded to form a "bdelloplast" in a type IV pilus-dependent process, and prey contents are digested, allowing Bdellovibrio growth and septation. After septation, Bdellovibrio flagellar motility resumes inside the prey bdelloplast prior to its lysis and escape of Bdellovibrio progeny. Bdellovibrio can also grow slowly outside prey as long flagellate host-independent (HI) cells, cultured on peptone-rich media. The B. bacteriovorus HD100 genome encodes three pairs of MotAB flagellar motor proteins, each of which could potentially form an inner membrane ion channel, interact with the FliG flagellar rotor ring, and produce flagellar rotation. In 2004, Flannagan and coworkers (R. S. Flannagan, M. A. Valvano, and S. F. Koval, Microbiology 150:649-656, 2004) used antisense RNA and green fluorescent protein (GFP) expression to downregulate a single Bdellovibrio motA gene and reported slowed release from the bdelloplast and altered motility of the progeny. Here we inactivated each pair of motAB genes and found that each pair contributes to motility, both predatorily, inside the bdelloplast and during HI growth; however, each pair was dispensable, and deletion of no pair abolished motility totally. Driving-ion studies with phenamil, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and different pH and sodium conditions indicated that all Mot pairs are proton driven, although the sequence similarities of each Mot pair suggests that some may originate from halophilic species. Thus, Bdellovibrio is a "dedicated motorist," retaining and expressing three pairs of mot genes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-10899118, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-11511869, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-12558595, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-12730325, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-1400154, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-14752164, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-14993314, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-15629949, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-1628843, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-16573680, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-17056298, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-17416646, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-17706914, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-18929071, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-19170881, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-19819245, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-20023029, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-20062540, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-3031432, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-4030690, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-4030691, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-6295879, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-6345791, http://linkedlifedata.com/resource/pubmed/commentcorrection/21148728-9917395
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1098-5530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
932-43
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Three motAB stator gene products in Bdellovibrio bacteriovorus contribute to motility of a single flagellum during predatory and prey-independent growth.
pubmed:affiliation
University of Nottingham, Nottingham NG7 2UH, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't