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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2011-3-1
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pubmed:abstractText |
Serine-threonine kinase receptor-associated protein (STRAP) functions as a regulator of both TGF-? and p53 signaling. However, the regulatory mechanism of STRAP activity is not understood. In this study, we report that B-MYB is a new STRAP-interacting protein, and that an amino-terminal DNA-binding domain and an area (amino acids 373-468) between the acidic and conserved regions of B-MYB mediate the B-MYB·STRAP interaction. Functionally, B-MYB enhances STRAP-mediated inhibition of TGF-? signaling pathways, such as apoptosis and growth inhibition, by modulating complex formation between the TGF-? receptor and SMAD3 or SMAD7. Furthermore, coexpression of B-MYB results in a dose-dependent increase in STRAP-mediated stimulation of p53-induced apoptosis and cell cycle arrest via direct interaction. Confocal microscopy showed that B-MYB prevents the normal translocation of SMAD3 in response to TGF-?1 and stimulates p53 nuclear translocation. These results suggest that B-MYB acts as a positive regulator of STRAP.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MDM2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MYBL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-mdm2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/SMAD3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SMAD7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/STRAP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Smad3 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Smad7 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/TP53 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1083-351X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
286
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7439-56
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pubmed:meshHeading |
pubmed-meshheading:21148321-Apoptosis,
pubmed-meshheading:21148321-Breast Neoplasms,
pubmed-meshheading:21148321-Cell Cycle Proteins,
pubmed-meshheading:21148321-Cell Nucleus,
pubmed-meshheading:21148321-Female,
pubmed-meshheading:21148321-HCT116 Cells,
pubmed-meshheading:21148321-HEK293 Cells,
pubmed-meshheading:21148321-HeLa Cells,
pubmed-meshheading:21148321-Hep G2 Cells,
pubmed-meshheading:21148321-Humans,
pubmed-meshheading:21148321-Neoplasm Proteins,
pubmed-meshheading:21148321-Protein Structure, Tertiary,
pubmed-meshheading:21148321-Proto-Oncogene Proteins c-mdm2,
pubmed-meshheading:21148321-RNA, Small Interfering,
pubmed-meshheading:21148321-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:21148321-Signal Transduction,
pubmed-meshheading:21148321-Smad3 Protein,
pubmed-meshheading:21148321-Smad7 Protein,
pubmed-meshheading:21148321-Trans-Activators,
pubmed-meshheading:21148321-Transforming Growth Factor beta,
pubmed-meshheading:21148321-Tumor Suppressor Protein p53
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pubmed:year |
2011
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pubmed:articleTitle |
B-MYB positively regulates serine-threonine kinase receptor-associated protein (STRAP) activity through direct interaction.
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pubmed:affiliation |
Department of Biochemistry, School of Life Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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