Linked Life Data

21147078

Source:http://linkedlifedata.com/resource/pubmed/id/21147078

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-1-24
pubmed:abstractText
Novaeguinoside II is one of the newly found asterosaponins, which are a kind of steroidal glycosides extracted from starfish with an anti-tumor effect. In this study, we investigated the bioactivity of novaeguinoside II on human U87MG glioblastoma cells. The results showed that novaeguinoside II significantly suppresses U87MG cell proliferation in a time- and concentration-dependent manner. Flow cytometric analysis of DNA in U87MG cells showed that novaeguinoside II induces the prominent appearance of an S phase peak in the cell cycle that is suggestive of apoptosis that is identical to the result of an annexin V/PI assay. Fluorescence and electron microscopy revealed apoptotic change of U87MG cells. The electrophoresis of DNA showed a typical "ladder" that is consistent with apoptotic DNA fragmentation. Cytofluorometry showed a decreased mitochondrial transmembrane potential in novaeguinoside II-treated U87MG cells. Western blot showed that novaeguinoside II increased the expression of cytochrome-c and caspase-3 protein. The novel findings suggest that novaeguinoside II can induce apoptosis of U87MG cells by a mitochondrial apoptotic pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1872-6240
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
4
pubmed:volume
1372
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Novaeguinoside II inhibits cell proliferation and induces apoptosis of human brain glioblastoma U87MG cells through the mitochondrial pathway.
pubmed:affiliation
Institute of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't