Source:http://linkedlifedata.com/resource/pubmed/id/21144838
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2011-1-10
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| pubmed:abstractText |
The novel plasmid vector (pTAOR4-Rev) suitable for gene expression in actinomycete strains of Pseudonocardia autotrophica was constructed from 2 P. autotrophica genetic elements, the novel replication origin and the acetone-inducible promoter. The replication origin was isolated from the endogenous plasmid of strain DSM 43082 and the acetone-inducible promoter was determined by analysis of the upstream region of an acetaldehyde dehydrogenase gene homologue in strain NBRC 12743. P. autotrophica strains transformed with pTAOR4-P450, carrying a gene for cytochrome P450 monooxygenase, expressed P450 from the acetone-inducible promoter, as verified by SDS-PAGE and spectral analysis. The biotransformation test of acetone-induced resting cells prepared from a strain of P. autotrophica carrying pTAOR4 that harbors a compactin (CP)-hydroxylating P450 gene revealed 3.3-fold increased production of pravastatin (PV), a drug for hypercholesterolemia. Biotransformation of CP by the same strain in batch culture yielded PV accumulation of 14.3 g/l after 100 h. The expression vector pTAOR4-Rev and its function-enhancing derivatives provide a versatile approach to industrial biotransformation by Pseudonocardia strains, which can be good hosts for P450 monooxygenase expression.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetone,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxymethylglutaryl-CoA...,
http://linkedlifedata.com/resource/pubmed/chemical/Lovastatin,
http://linkedlifedata.com/resource/pubmed/chemical/Pravastatin,
http://linkedlifedata.com/resource/pubmed/chemical/compactin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1090-2104
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
7
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| pubmed:volume |
404
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
511-6
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| pubmed:meshHeading |
pubmed-meshheading:21144838-Acetone,
pubmed-meshheading:21144838-Base Sequence,
pubmed-meshheading:21144838-Biotransformation,
pubmed-meshheading:21144838-Cloning, Molecular,
pubmed-meshheading:21144838-Cytochrome P-450 Enzyme System,
pubmed-meshheading:21144838-Gene Expression,
pubmed-meshheading:21144838-Genetic Engineering,
pubmed-meshheading:21144838-Genetic Vectors,
pubmed-meshheading:21144838-Hydroxymethylglutaryl-CoA Reductase Inhibitors,
pubmed-meshheading:21144838-Industrial Microbiology,
pubmed-meshheading:21144838-Lovastatin,
pubmed-meshheading:21144838-Molecular Sequence Data,
pubmed-meshheading:21144838-Plasmids,
pubmed-meshheading:21144838-Pravastatin,
pubmed-meshheading:21144838-Promoter Regions, Genetic,
pubmed-meshheading:21144838-Replication Origin,
pubmed-meshheading:21144838-Streptomyces
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| pubmed:year |
2011
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| pubmed:articleTitle |
Construction of a novel expression vector in Pseudonocardia autotrophica and its application to efficient biotransformation of compactin to pravastatin, a specific HMG-CoA reductase inhibitor.
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| pubmed:affiliation |
Bioresource Laboratories, Mercian Corporation, 1808 Nakaizumi, Iwata, Shizuoka 438-0078, Japan.
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| pubmed:publicationType |
Journal Article
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