Linked Life Data

2113803

Source:http://linkedlifedata.com/resource/pubmed/id/2113803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-7-31
pubmed:abstractText
The immunocytochemical and immunochemical identification of beta-protein precursor in cultured vascular cells was undertaken using three types of antibodies to the synthetic predictive peptides (extracellular portion; 275-286, beta-protein portion; 597-620, C-terminal portion; 681-695) of beta-protein precursor. Monoclonal antibody directed toward the beta-protein portion stained the surface membrane of the cultured endothelial cells as well as cytoplasmic organelles. Immunoblotting of the subcellular fractions of the cell homogenate with three antibodies revealed that the plasma membrane associated beta-protein precursor consists of 105-130 kDa protein complexes and that the mitochondria-microsome-associated beta-protein consists of related 30-67 kDa protein complexes. The immunoreactivity of the protein bands was blocked by pretreating the monoclonal antibody with bovine serum albumin-conjugated beta-protein. These results indicate that the form of the beta-protein precursor or beta-protein-related components in cultured vascular cells is heterogeneous in terms of molecular size and subcellular localization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0098-6127
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-32
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Immunocytochemical identification of beta-protein precursor in cultured vascular cells.
pubmed:affiliation
Department of Biology, Tokyo Metropolitan Institute of Gerontology.
pubmed:publicationType
Journal Article