Source:http://linkedlifedata.com/resource/pubmed/id/21135815
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2011-3-30
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pubmed:abstractText |
Pruritus, also known as itch, is a sensation that causes a desire to scratch. Prolonged scratching exacerbates skin lesions in several skin diseases such as atopic dermatitis. Here, we identify the cystic fibrosis transmembrane conductance regulator (CFTR/Cftr), an integral membrane protein that mediates transepithelial chloride transport, as a determinant factor in mice for the susceptibility to several cutaneous symptoms during mite infestation. Mice that endogenously express dysfunctional Cftr (Cftr(?F508/?F508)) show significant increase of scratching behavior and skin fibrosis after mite exposure. These phenotypes were due to the increased expression of nerve growth factor (NGF) that augments the sensitization of peripheral nerve fibers. Moreover, protein gene product 9.5 (PGP9.5)-positive neurites were abundant in the epidermis of mite-infested Cftr(?F508/?F508) mice. Furthermore, mite-infested Cftr(+/+) mice orally administered with a chloride channel inhibitor glibenclamide had higher scratching count and increased level of NGF than vehicle-treated mice. Consistently, mite extract-exposed primary and transformed human keratinocytes, treated with CFTR inhibitor, had significantly higher level of NGF mRNA compared with vehicle-treated, mite extract-exposed cells. These results reveal that CFTR in keratinocytes plays a critical role for the regulation of peripheral nerve function and pruritus sensation, and suggest that Cftr(?F508/?F508) mice may serve as a novel mouse model that represents NGF-dependent generation of pruritus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Cystic Fibrosis Transmembrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Glyburide,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1530-0307
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pubmed:author |
pubmed-author:HashimotoYasuakiY,
pubmed-author:KaiHirofumiH,
pubmed-author:KogaTomoakiT,
pubmed-author:MizunoeShotaS,
pubmed-author:NiiboriAkikoA,
pubmed-author:SatoTakashiT,
pubmed-author:ScholteBobB,
pubmed-author:ShimasakiShogoS,
pubmed-author:ShutoTsuyoshiT,
pubmed-author:SugaharaTakuyaT,
pubmed-author:SugiyamaTakashiT,
pubmed-author:SuicoMary AnnMA,
pubmed-author:TakeyaMotohiroM,
pubmed-author:TomitaAzusaA
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pubmed:issnType |
Electronic
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
509-18
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pubmed:meshHeading |
pubmed-meshheading:21135815-Animals,
pubmed-meshheading:21135815-Cells, Cultured,
pubmed-meshheading:21135815-Chloride Channels,
pubmed-meshheading:21135815-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:21135815-Disease Susceptibility,
pubmed-meshheading:21135815-Fibrosis,
pubmed-meshheading:21135815-Glyburide,
pubmed-meshheading:21135815-Humans,
pubmed-meshheading:21135815-Keratinocytes,
pubmed-meshheading:21135815-Mice,
pubmed-meshheading:21135815-Mite Infestations,
pubmed-meshheading:21135815-Nerve Growth Factor,
pubmed-meshheading:21135815-Neurites,
pubmed-meshheading:21135815-Peripheral Nerves,
pubmed-meshheading:21135815-Phenotype,
pubmed-meshheading:21135815-Pruritus,
pubmed-meshheading:21135815-RNA, Messenger,
pubmed-meshheading:21135815-Skin
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pubmed:year |
2011
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pubmed:articleTitle |
CFTR-deficiency renders mice highly susceptible to cutaneous symptoms during mite infestation.
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pubmed:affiliation |
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE Cell Fate Regulation Research and Education Unit, Kumamoto University, Kumamoto, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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