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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2011-1-6
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pubmed:abstractText |
RNA interference (RNAi) emerges as a powerful tool to induce loss-of-function phenotypes. In the context of the brain, gene manipulation is best targeted to specific subsets of cells in order to achieve a physiologically relevant outcome. Polymerase II-based viral expression systems can be used to cell-specifically express constructs incorporating flanking and loop sequences from endogenous microRNA (miRNA), which directs the designed hairpins into the endogenous gene silencing machinery. While many studies have documented non-cell-selective gene knock-down in the brain, it has not been tested whether different cell types or different areas of the central nervous system (CNS) are equally amenable to this approach. We have evaluated this issue using a tetracycline (Tet)-controllable and cell-specific miRNA 30 (miR30)-based short hairpin (shRNA) interference system.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21134262-10933976,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2199
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93
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pubmed:dateRevised |
2011-7-26
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pubmed:meshHeading |
pubmed-meshheading:21134262-Animals,
pubmed-meshheading:21134262-Brain,
pubmed-meshheading:21134262-Brain Stem,
pubmed-meshheading:21134262-Cell Line,
pubmed-meshheading:21134262-Gene Knockdown Techniques,
pubmed-meshheading:21134262-Genetic Vectors,
pubmed-meshheading:21134262-Hippocampus,
pubmed-meshheading:21134262-MicroRNAs,
pubmed-meshheading:21134262-Nitric Oxide Synthase,
pubmed-meshheading:21134262-RNA, Small Interfering,
pubmed-meshheading:21134262-RNA Interference,
pubmed-meshheading:21134262-Rats
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pubmed:year |
2010
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pubmed:articleTitle |
Cell- and region-specific miR30-based gene knock-down with temporal control in the rat brain.
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pubmed:affiliation |
Department of Physiology and Pharmacology, Bristol Heart Institute, University of Bristol, UK. bh.liu@bristol.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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