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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-7-26
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pubmed:abstractText |
The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 - 2 x 10(-10) cm2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
602-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2113381-Animals,
pubmed-meshheading:2113381-Biological Transport,
pubmed-meshheading:2113381-Cell Survival,
pubmed-meshheading:2113381-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2113381-Endocytosis,
pubmed-meshheading:2113381-Fluorescein-5-isothiocyanate,
pubmed-meshheading:2113381-Fluoresceins,
pubmed-meshheading:2113381-Fluorescent Dyes,
pubmed-meshheading:2113381-Hemagglutination Tests,
pubmed-meshheading:2113381-Humans,
pubmed-meshheading:2113381-Kinetics,
pubmed-meshheading:2113381-Liver Neoplasms, Experimental,
pubmed-meshheading:2113381-Macromolecular Substances,
pubmed-meshheading:2113381-Protein Binding,
pubmed-meshheading:2113381-Rats,
pubmed-meshheading:2113381-Ricin,
pubmed-meshheading:2113381-Thiocyanates,
pubmed-meshheading:2113381-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Binding and internalization of ricin labelled with fluorescein isothiocyanate.
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pubmed:affiliation |
Dipartimento di Scienze Biochimiche e Centro di Biologia Molecolare del C.N.R., Universita di Roma La Sapienza, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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