Linked Life Data

21133355

Source:http://linkedlifedata.com/resource/pubmed/id/21133355

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-12
pubmed:abstractText
We investigate the cooperative effect of molecular tilt and defocus on fluorophore localization by centroid calculation in far-field superresolution microscopy based on stochastic single molecule switching. If tilt angle and defocus are unknown, the localization contains systematic errors up to about ±125 nm. When imaging rotation-impaired fluorophores of unknown random orientation, the average localization accuracy in three-dimensional samples is typically limited to about ±32 nm, restricting the attainable resolution accordingly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1530-6992
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-13
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy.
pubmed:affiliation
German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. shell@gwdg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't