Source:http://linkedlifedata.com/resource/pubmed/id/21132425
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2011-2-11
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| pubmed:abstractText |
We evaluated a large collection of Tos17 mutant panel lines for their reaction to three different races of Magnaporthe oryzae and identified a lesion mimic mutant, NF4050-8, that showed lesions similar to naturally occurring spl5 mutant and enhanced resistance to all the three blast races tested. Nested modified-AFLP using Tos17-specific primers and southern hybridization experiments of segregating individuals indicated that the lesion mimic phenotype in NF4050-8 is most likely due to a nucleotide change acquired during the culturing process and not due to Tos17 insertion per se. Inheritance and genetic analyses in two japonica × indica populations identified an overlapping genomic region of 13 cM on short arm of chromosome 7 that was linked with the lesion mimic phenotype. High-resolution genetic mapping using 950 F(3) and 3,821 F(4) plants of NF4050-8 × CO39 delimited a 35 kb region flanked by NBARC1 (5.262 Mb) and RM8262 (5.297 Mb), which contained 6 ORFs; 3 of them were 'resistance gene related' with typical NBS-LRR signatures. One of them harbored a NB-ARC domain, which had been previously demonstrated to be associated with cell death in animals. Microarray analysis of NF4050-8 revealed significant up-regulation of numerous defense/pathogenesis-related genes and down-regulation of heme peroxidase genes. Real-time PCR analysis of WRKY45 and PR1b genes suggested possible constitutive activation of a defense signaling pathway downstream of salicylic acid but independent of NH1 in these mutant lines of rice.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1432-2242
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
122
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
831-54
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| pubmed:meshHeading |
pubmed-meshheading:21132425-Alleles,
pubmed-meshheading:21132425-Chromosome Segregation,
pubmed-meshheading:21132425-Chromosomes, Plant,
pubmed-meshheading:21132425-Crosses, Genetic,
pubmed-meshheading:21132425-Gene Expression Profiling,
pubmed-meshheading:21132425-Gene Expression Regulation, Plant,
pubmed-meshheading:21132425-Gene Library,
pubmed-meshheading:21132425-Genes, Plant,
pubmed-meshheading:21132425-Genetic Linkage,
pubmed-meshheading:21132425-Genetic Loci,
pubmed-meshheading:21132425-Genotype,
pubmed-meshheading:21132425-Immunity, Innate,
pubmed-meshheading:21132425-Inheritance Patterns,
pubmed-meshheading:21132425-Japan,
pubmed-meshheading:21132425-Magnaporthe,
pubmed-meshheading:21132425-Molecular Sequence Annotation,
pubmed-meshheading:21132425-Mutagenesis, Insertional,
pubmed-meshheading:21132425-Mutation,
pubmed-meshheading:21132425-Nucleotides,
pubmed-meshheading:21132425-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:21132425-Oryza sativa,
pubmed-meshheading:21132425-Physical Chromosome Mapping,
pubmed-meshheading:21132425-Plant Diseases,
pubmed-meshheading:21132425-Regeneration,
pubmed-meshheading:21132425-Tissue Culture Techniques
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| pubmed:year |
2011
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| pubmed:articleTitle |
Isolation, fine mapping and expression profiling of a lesion mimic genotype, spl(NF4050-8) that confers blast resistance in rice.
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| pubmed:affiliation |
National Institute of Agrobiosciences (NIAS), Tsukuba, Ibaraki, Japan. r.babu@cgiar.org
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| pubmed:publicationType |
Journal Article
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