|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2011-1-6
|
|
pubmed:abstractText |
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
1545-9985
|
|
pubmed:author |
pubmed-author:AhringerJulieJ,
pubmed-author:AlekseyenkoArtyom AAA,
pubmed-author:CheungMing-SinMS,
pubmed-author:DayDaniel SDS,
pubmed-author:EgelhoferThea ATA,
pubmed-author:ElginSarah C RSC,
pubmed-author:GadelSarahS,
pubmed-author:GorchakovAndrey AAA,
pubmed-author:GuTingtingT,
pubmed-author:HawkinsR DavidRD,
pubmed-author:KarpenGary HGH,
pubmed-author:KharchenkoPeter VPV,
pubmed-author:KlugmanSaritS,
pubmed-author:Kolasinska-ZwierzPaulinaP,
pubmed-author:KuanSamanthaS,
pubmed-author:KurodaMitzi IMI,
pubmed-author:LatorreIsabelI,
pubmed-author:LeeKyungjoonK,
pubmed-author:LiebJason DJD,
pubmed-author:Linder-BassoDanielaD,
pubmed-author:LuuYingY,
pubmed-author:MinodaAkiA,
pubmed-author:NgoQueminhQ,
pubmed-author:ParkPeter JPJ,
pubmed-author:PerryMarcM,
pubmed-author:PirrottaVincenzoV,
pubmed-author:RechtsteinerAndreasA,
pubmed-author:RenBingB,
pubmed-author:RiddleNicole CNC,
pubmed-author:SchwartzYuri BYB,
pubmed-author:ShanowerGregory AGA,
pubmed-author:StromeSusanS,
pubmed-author:VielleAnneA
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
18
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
91-3
|
|
pubmed:dateRevised |
2011-8-1
|
|
pubmed:meshHeading |
pubmed-meshheading:21131980-Animals,
pubmed-meshheading:21131980-Antibodies,
pubmed-meshheading:21131980-Antibody Specificity,
pubmed-meshheading:21131980-Blotting, Western,
pubmed-meshheading:21131980-Caenorhabditis elegans,
pubmed-meshheading:21131980-Caenorhabditis elegans Proteins,
pubmed-meshheading:21131980-Chromatin Immunoprecipitation,
pubmed-meshheading:21131980-Drosophila Proteins,
pubmed-meshheading:21131980-Drosophila melanogaster,
pubmed-meshheading:21131980-Histones,
pubmed-meshheading:21131980-Immunoblotting,
pubmed-meshheading:21131980-Protein Processing, Post-Translational,
pubmed-meshheading:21131980-Quality Control,
pubmed-meshheading:21131980-Reproducibility of Results
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
An assessment of histone-modification antibody quality.
|
|
pubmed:affiliation |
Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
