Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-4-25
pubmed:abstractText
To investigate the potential of nanoparticles (NPs) composed of poly(?-glutamic acid) conjugated with l-phenylalanine (?-PGA-Phe NPs) for the treatment of retinal diseases,? ?-PGA-Phe NPs (200nm) were tested with macrophages and microglia in vitro or by intravitreal administration into normal or pathological rat eyes. The anti-inflammatory effects of the NPs containing dexamethasone (DEX-NPs) were examined using qRT-PCR in vitro by counting activated microglia and Fluorogold-labeled retinal ganglion cells in the retinas under excitotoxicity or by counting TUNEL (+) photoreceptors in the detached retinas. The NPs were taken up efficiently by cultured macrophages or microglia. At day 7, 60-80% of the diffuse signal remained in the cytoplasm of these cells. In normal rat eyes, the NPs did not accumulate in the retinas and no inflammatory cells were recruited. Conversely, under pathological conditions, the NPs were localized in activated CD11b-positive cells in the retina. DEX-NPs suppressed the expression of TNF? and MCP-1 in cultured macrophages or microglia, the activation of microglia, the loss of retinal ganglion cells (RGCs) in excitotoxic retinas, and the number of TUNEL (+) photoreceptors in detached retinas. These data suggest that ?-PGA-Phe NPs can be a powerful tool for suppressing inflammatory cells in pathological conditions in the retina.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-4995
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
151
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
65-73
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Suppression of phagocytic cells in retinal disorders using amphiphilic poly(?-glutamic acid) nanoparticles containing dexamethasone.
pubmed:affiliation
Department of Ophthalmology, Tohoku Graduate University School of Medicine, 1-1 Seiryo, Aoba, Sendai, Miyagi 980-8574, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't