Source:http://linkedlifedata.com/resource/pubmed/id/21127173
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2011-3-4
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| pubmed:abstractText |
In the hematopoietic hierarchy, only stem cells are thought to be capable of long-term self-renewal. Erythroid progenitors derived from fetal or adult mammalian hematopoietic tissues are capable of short-term, or restricted (10(2)- to 10(5)-fold), ex vivo expansion in the presence of erythropoietin, stem cell factor, and dexamethasone. Here, we report that primary erythroid precursors derived from early mouse embryos are capable of extensive (10(6)- to 10(60)-fold) ex vivo proliferation. These cells morphologically, immunophenotypically, and functionally resemble proerythroblasts, maintaining both cytokine dependence and the potential, despite prolonged culture, to generate enucleated erythrocytes after 3-4 maturational cell divisions. This capacity for extensive erythroblast self-renewal is temporally associated with the emergence of definitive erythropoiesis in the yolk sac and its transition to the fetal liver. In contrast, hematopoietic stem cell-derived definitive erythropoiesis in the adult is associated almost exclusively with restricted ex vivo self-renewal. Primary primitive erythroid precursors, which lack significant expression of Kit and glucocorticoid receptors, lack ex vivo self-renewal capacity. Extensively self-renewing erythroblasts, despite their near complete maturity within the hematopoietic hierarchy, may ultimately serve as a renewable source of red cells for transfusion therapy.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid,
http://linkedlifedata.com/resource/pubmed/chemical/hydrocortisone receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1528-0020
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| pubmed:author | |
| pubmed:copyrightInfo |
© 2011 by The American Society of Hematology
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
3
|
| pubmed:volume |
117
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2708-17
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| pubmed:dateRevised |
2011-5-16
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| pubmed:meshHeading |
pubmed-meshheading:21127173-Animals,
pubmed-meshheading:21127173-Cell Differentiation,
pubmed-meshheading:21127173-Cell Division,
pubmed-meshheading:21127173-Cell Proliferation,
pubmed-meshheading:21127173-Cell Size,
pubmed-meshheading:21127173-Cell Survival,
pubmed-meshheading:21127173-Cells, Cultured,
pubmed-meshheading:21127173-Cytokines,
pubmed-meshheading:21127173-Dexamethasone,
pubmed-meshheading:21127173-Erythroblasts,
pubmed-meshheading:21127173-Fetus,
pubmed-meshheading:21127173-Humans,
pubmed-meshheading:21127173-Liver,
pubmed-meshheading:21127173-Mammals,
pubmed-meshheading:21127173-Mice,
pubmed-meshheading:21127173-Mice, Inbred C57BL,
pubmed-meshheading:21127173-Phenotype,
pubmed-meshheading:21127173-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:21127173-Receptors, Erythropoietin,
pubmed-meshheading:21127173-Receptors, Glucocorticoid,
pubmed-meshheading:21127173-Stem Cells,
pubmed-meshheading:21127173-Yolk Sac
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| pubmed:year |
2011
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| pubmed:articleTitle |
Immature erythroblasts with extensive ex vivo self-renewal capacity emerge from the early mammalian fetus.
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| pubmed:affiliation |
Department of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester Medical Center, Rochester, NY 14642, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|