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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0024432,
umls-concept:C0056912,
umls-concept:C0127400,
umls-concept:C0205225,
umls-concept:C0243144,
umls-concept:C0439855,
umls-concept:C0678226,
umls-concept:C0916194,
umls-concept:C1327414,
umls-concept:C1539102,
umls-concept:C2349975
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pubmed:issue |
1
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pubmed:dateCreated |
2011-1-19
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pubmed:abstractText |
Unmethylated CpG sequences (CpG DNA) can induce Th1 response and thus become a potential immunotherapeutic agent. A key step in the treatment is to transport CpG DNA to its receptor TLR9 located in the endocytosis pathway of target immune cells. For the effective transport, we prepared a novel complex from a ?-1,3-glucan schizophyllan (SPG) and CpG DNA, and administered the complex to murine peritoneal macrophages that had been previously activated by thioglycollate and expressed a major ?-1,3-glucan receptor Dectin-1 on the cellular surface. Flow cytometric analysis and microscopic observation showed that the complex was taken up by the macrophage through Dectin-1 mediated pathway. Indeed, ELISA demonstrated that IL-12 production was increased sigmoidally with increasing SPG/CpG DNA ratio in the complexation, and reached the maximum at the SPG-rich composition. In the present work, we describe a new approach to deliver CpG DNA to immune cells by use of a ?-1,3-glucan/DNA complex.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1520-4812
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
19
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-15
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pubmed:meshHeading |
pubmed-meshheading:21126031-Animals,
pubmed-meshheading:21126031-Base Sequence,
pubmed-meshheading:21126031-Biological Transport,
pubmed-meshheading:21126031-CpG Islands,
pubmed-meshheading:21126031-Cytokines,
pubmed-meshheading:21126031-DNA,
pubmed-meshheading:21126031-Drug Carriers,
pubmed-meshheading:21126031-Gene Expression Regulation,
pubmed-meshheading:21126031-HEK293 Cells,
pubmed-meshheading:21126031-Humans,
pubmed-meshheading:21126031-Interleukin-12,
pubmed-meshheading:21126031-Macrophages,
pubmed-meshheading:21126031-Membrane Proteins,
pubmed-meshheading:21126031-Mice,
pubmed-meshheading:21126031-Models, Molecular,
pubmed-meshheading:21126031-Nerve Tissue Proteins,
pubmed-meshheading:21126031-Nucleic Acid Conformation,
pubmed-meshheading:21126031-Sizofiran
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pubmed:year |
2011
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pubmed:articleTitle |
Enhanced cytokine secretion from primary macrophages due to Dectin-1 mediated uptake of CpG DNA/?-1,3-glucan complex.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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