Microarray technology is limited to monitoring the expression of previously annotated genes that have corresponding probes on the array. Computationally annotated genes have not fully been validated, because ESTs and full-length cDNAs cannot cover entire transcribed regions. Here, mRNA-Seq (an Illumina cDNA sequencing application) was used to monitor whole mRNAs of salinity stress-treated rice tissues.
National Institute of Agrobiological Sciences (NIAS), Division of Genome and Biodiversity Research, 1-2 Kannondai 2-chome, Tsukuba, Ibaraki 305-8602, Japan.
