Source:http://linkedlifedata.com/resource/pubmed/id/21118722
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-1-31
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| pubmed:abstractText |
The targeting potential of OX-26-decorated immunoliposomes was investigated, using the human brain endothelial cell line hCMEC/D3 as a model of the blood-brain barrier (BBB). Immuno-nanoliposomes were prepared by the biotin/streptavidin ligation strategy, and their uptake by hCMEC/D3 cells and permeability through cell monolayers was studied. In order to elucidate the mechanisms of uptake, pH-sensitive fluorescence signal of HPTS was used, while transport was measured using double labeled immunoliposomes (with aqueous and lipid membrane fluorescent tags). PEGylated and non-specific-IgG-decorated liposomes were studied under identical conditions, as controls. CHO-K1 cells (which do not overexpress the transferrin receptor) were studied in some cases for comparative purposes. Experimental results reveal that hCMEC/D3 cells are good models for in vitro screening of BBB-targeting nanoparticulate drug delivery systems. Uptake and transcytosis of immunoliposome-associated dyes by cell monolayers was substantially higher compared to those of control liposomes. HPTS-entrapping OX-26-immunoliposome uptake indicated lysosomal localization and receptor-mediated mechanism. The ratio of aqueous/lipid label transport is affected by pre-incubation with antibody, or use of high lipid doses, suggesting that vesicles are transported intact after lysosome saturation. Co-decoration with a second ligand slightly decreases OX-26-decorated vesicle uptake, but not transcytosis, proving that the biotin-streptavidin technique can be applied for the generation of dual-targeting nanoliposomes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/Transferrin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1873-3441
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
77
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
265-74
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:21118722-Animals,
pubmed-meshheading:21118722-Antibodies, Monoclonal,
pubmed-meshheading:21118722-Biological Transport,
pubmed-meshheading:21118722-Blood-Brain Barrier,
pubmed-meshheading:21118722-Cell Line,
pubmed-meshheading:21118722-Cell Survival,
pubmed-meshheading:21118722-Cells, Cultured,
pubmed-meshheading:21118722-Endothelial Cells,
pubmed-meshheading:21118722-Humans,
pubmed-meshheading:21118722-Immunoglobulin G,
pubmed-meshheading:21118722-Liposomes,
pubmed-meshheading:21118722-Mice,
pubmed-meshheading:21118722-Nanoparticles,
pubmed-meshheading:21118722-Particle Size,
pubmed-meshheading:21118722-Permeability,
pubmed-meshheading:21118722-Physicochemical Phenomena,
pubmed-meshheading:21118722-Receptors, Transferrin,
pubmed-meshheading:21118722-Transcytosis,
pubmed-meshheading:21118722-Transferrin
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| pubmed:year |
2011
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| pubmed:articleTitle |
Uptake and permeability studies of BBB-targeting immunoliposomes using the hCMEC/D3 cell line.
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| pubmed:affiliation |
Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Rio, Greece.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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