Linked Life Data

21116968

Source:http://linkedlifedata.com/resource/pubmed/id/21116968

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-11-30
pubmed:abstractText
This chapter describes a method for the analysis of human hepatocarcinoma cells (Hep G2 cells) for reduced and oxidized glutathione, following treatment with nanoparticle formulations. Glutathione is a tripeptide (L-?-glutamyl-L-cysteinyl-glycine) present intracellularly in millimolar concentrations and one of the primary cellular antioxidant defenses against oxidative stress. An increase in the relative amount of oxidized to reduced glutathione may be indicative of oxidative stress, while a decrease in the overall glutathione pool may be indicative of conjugative metabolism or impaired synthesis. The method presented in this chapter utilizes a colorimetric method for detection of reduced and oxidized glutathione.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
697
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
191-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Monitoring glutathione homeostasis in nanoparticle-treated hepatocytes.
pubmed:affiliation
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural