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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1990-6-25
|
| pubmed:abstractText |
Diphtheritic bacteria of PW-8 Massachusetts strain produced into cultural medium only one nucleotidase--endoDNAase. The enzyme was synthesized by the cells during the exponential phase of growth. The DNAase was purified 500-fold and exhibited properties specific to neutral-alkaline DNAases (pH optimum about 7.5, absolute requirements for Me2+, single-step mechanism of substrate hydrolysis). The following properties were typical for the enzyme: absence of distinct specificity to structure of bases surrounding the hydrolyzed bond, formation of 5'-end phosphate groups and slightly higher preference to denatured DNA.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0042-8809
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
27-31
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2111600-Corynebacterium diphtheriae,
pubmed-meshheading:2111600-DNA, Bacterial,
pubmed-meshheading:2111600-Deoxyribonucleases,
pubmed-meshheading:2111600-Enzyme Activation,
pubmed-meshheading:2111600-Hydrogen-Ion Concentration,
pubmed-meshheading:2111600-Hydrolysis,
pubmed-meshheading:2111600-Kinetics,
pubmed-meshheading:2111600-Viscosity
|
| pubmed:articleTitle |
[Deoxyribonuclease from Corynebacterium diphtheriae: dynamics of synthesis and properties].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|